Imaging and studying human topoisomerase I on mica surfaces in air and in liquid by atomic force microscopy |
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Authors: | Zhiguo Liu Ronghua Meng Yuangang Zu Qingyong Li Liping Yao |
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Affiliation: | Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin, China |
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Abstract: | In this study, the topography of human topoisomerase I (TOPO I) on mica surfaces in air and in liquid has been studied by atomic force microscopy (AFM). The average height of TOPO I on mica surface in air measured by AFM was 2.59±0.32 nm. After adsorption of the 0.3 U/µl TOPO I on mica surfaces for 2 h, and then imaged in liquid by AFM, well‐separated single TOPO I was observed. The average height of TOPO I on mica surfaces in liquid measured by AFM was 2.93±0.42 nm. After adsorption of the 4 U/µl TOPO I on mica surfaces for 1.5 h, TOPO I monolayer can be formed. The produced TOPO I monolayer on mica was flat and exhibited good stability. SCANNING 31: 160–166, 2009. © 2009 Wiley Periodicals, Inc. |
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Keywords: | atomic force microscopy (AFM) topoisomerase I (TOPO I) mica monolayer |
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