使用生物反应器酶促合成酸酐（英）

用装有固定化脂肪酶的生物反应器系统对有效地合成蓖麻油酸酐进行了尝试。合成酸酐试图证明：通过控制反应混合物的最适含水量,反应是否能达到目标的缩合度。结果表明,使用生物反应器,反应能迅速地进行,在生物反应器内进行重复间歇反应是可行的,这样与不同脂肪酶相比较,每次脂肪酶的用量均能大大减少。

Enzymatic Synthesis of Estolides by a Bioreactor

An attempt was made to synthesize ricinoleic acid estolides effectively with a bioreactor system containing immobilized lipase. The estolide synthesis was attempted to confirm if the reaction would have proceeded to the targeted degree of condensation by controlling the reaction mixture under the optimal water content.The result showed that,with the bioreactor, the reaction could proceed rapidly.A repeated batch reaction was possible in the bioreactor.The amount of lipase used can thus be highly reduced compared with discarding it each time. The loading density of enzyme onto the carrier greatly affected enzymatic activity,with a loading level of 60mg lipase/g carrier producing 60% more estolide per gram of enzyme than a loading level of 120mg/g .