响应面优化超声波辅助提取发芽糙米黄酮工艺

以体积分数50%的乙醇溶液为提取溶剂，采用超声技术从发芽糙米中提取黄酮类化合物。采用Box-Behnken响应面设计法建立影响因素的二次回归模型。通过响应面分析得到超声波辅助提取发芽糙米黄酮的最佳提取条件为超声时间11.7min、浸提温度45.8℃、浸提时间30.5min、液料比18.9:1(mL/g)，发芽糙米黄酮的提取量可达(0.743±0.011)mg/g(n＝5)，达到理论值0.754mg/g的98.5%。该回归模型模拟度良好，可以作为发芽糙米黄酮提取量测定的理论依据。

Optimization of Ultrasound-Assisted Extraction of Flavonoids from Germinated Brown Rice Using Response Surface Methodology

Total flavonoids were extracted from geminated brown rice by ultrasound-assisted extraction method using 50% ethanol as extraction solvent.Response surface methodology was employed to optimize the extraction conditions based on extraction rate.A quadratic regression model describing the effects of four process conditions on the extraction rate of total flavonoids was developed using a Box-Behnken experimental design.The response surface analysis showed that the optimal extraction conditions were extraction for 30.5 min at 45.8 ℃ and a material/liquid ratio of 18.9:1(mL/g) after 11.7 min of ultrasound treatment.Under these conditions,the average extraction rate of total flavonoids was(0.743 ± 0.011) mg/g(n = 5),accounting for 98.5% of the predicted value,0.754 mg/g.The developed regression model showed excellent goodness of fit.