不锈钢丝SPME-HPLC联用测定水样中的芳香烃

在修饰过的不锈钢丝表面涂覆(苯乙烯-二乙烯基苯)高内相乳液,制作了不锈钢丝固相微萃取(SPME)萃取头,与高效液相色谱(HPLC)联用测定了环境水样中的芳香烃。系统考察了萃取时间及解吸时间等实验条件对萃取效率的影响,建立了测定环境水样中芳香烃的SPME-HPLC方法。实验结果表明,方法的柱内(n=6)和柱间(n=3)精密度(RSD)分别小于6.5%和8.6%,实际样品的加标回收率在84.4%~95.4%之间;3种芳香烃化合物的线性范围为5~200μg/L,线性相关系数r>0.9960,检出限在0.09~0.20μg/L之间。该方法快速、灵敏、简便,适用于快速分析环境水样中的芳香烃。

A Novel Coated Stainless Steel Fiber for SPME-HPLC of Aromatic Hydrocarbons in Waters

A novel method was used in the preparation of a solid -phase microextraction （ SPME ） coating , where styrene-divinyl benzene high internal phase emulsion was directly bonded to the surface of a modified stainless steel wire .Coupled to high performance liquid chromatography （ HPLC ） , the prepared coating was applied in the extraction and determination of three aromatic hydrocarbons in environmental waters.The experimental conditions of SPME , such as extraction time and desorption time were optimized.As a result , the run-to-run （ n=6 ） and column -to-column （ n=3） relative standard deviations of this method were lower than 6.5%and 8.6%, respectively, the recoveries of three aromatic hydrocarbons were ranged from 84.4%-95.4%, the linear relationships for three aromatic hydrocarbons were obtained with 〉0.9960 （5-200μg/L）, and limits of detection ranged from 0.09 to 0.20μg/L after optimization of all extraction steps.This method was rapid, sensitive, simple and suitable for rapid analysis of aromatic hydrocarbons in environmental water samples.