高效液相色谱法同时测定酱油中的苯甲酸和山梨酸

探讨了采用高效液相色谱法(HPLC)同时测定酱油中的苯甲酸、山梨酸的分析方法。色谱条件:流动相为甲醇与乙酸铵溶液(0.02 mol/L),体积比为5∶95,流速1.0 mL/min,紫外检测波长230 nm,柱温25℃,进样体积10μL。采用外标法定量,在5~100μg/mL,苯甲酸、山梨酸回归方程相关系数>0.999。检出限分别为0.35μg/mL和0.32μg/mL,平均回收率分别为94.09%和98.15%,相对标准偏差(RSD)在2.09%~3.90%。实验结果表明,该方法准确、灵敏、操作方便。

Study on simultaneous determination of benzoic acid and sorbic acid in soy by HPLC

The analysis method of simultaneous determination of benzoic acid and sorbic acid in soy was discussed by HPLC.Chromatographic conditions were as follows: methanol-0.02 mol/L NH4Ac(5∶95)as the mobile phase,flow rate is 1.0 mL/min,UV wave length of detection is 230 nm,column temperature is 25 ℃ and injection volume is 10 μL.A good linear relationship was obtained by the external standard method in the range of 5～100 μg/mL.The correlation coefficient of regression equation of standard sample were more than 0.999.The detection limit of benzoic acid and sorbic acid were 0.35μg/mL,0.32μg/mL.The average recovery were 94.09% and 98.15%,respectively.The relative standard deviation were 2.09%~3.90%.It shows that this method is accurate,sensitive and simple.