蔬菜中20种有机氯类农药残留气相色谱分析方法的研究

对国标气相色谱法检测蔬菜中20种有机氯农药残留的方法做了改进,试样前处理改用乙腈溶剂在弗洛里硅土固相萃取柱上净化;使用CP-Sil 24CB毛细管色谱柱分离,ECD测定。结果表明,在所建立的分析下,20种有机氯农药残留能够很好分离。工作曲线有良好的线性。检出限在0.1～0.8μg/kg范围内,回收率在80.5%～105.6%之间,方法的变异系数为0.36%～12.9%。该法简单、快捷、灵敏、准确,满足日常蔬菜中20种有机氯农药残留的常规分析检测要求。建立的改进方法适合大宗试样的分析。

Determination of 20 Organochlorine Pesticide Residuals in Vegetables by Gas Chromatography

An analytical metod has been developed for the determination of 20 organochlorine in vegetables.The sample was deterined by electron capture detector(ECD) with CP-Sil 24CB capillary column after acetonitrile extraction,salting-out,concentration,Florisil solid phase extraction column purification,and dissolving to constant volume with n-hexane.The results indicated that the 20 organochlorine were well separated.The recoveries were 80.5%～105.6%,and the coefficient of variation were 0.36%～12.9%.The detection limits were 0.1～0.8 μg/kg.The linear relationship and recovery results were satisfactory.The method is simple, rapid,accurate and highly sensitive,and it can be used for the simultaneous determination of 20 organochlorine in vegetables.