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| IEC-6细胞的培养鉴定和生长特性研究 | |
| 引用本文： | 陈祥贵,芮光伟,任川洪,王瑞淑,王志民,冉新泽. IEC-6细胞的培养鉴定和生长特性研究[J]. 西华大学学报(自然科学版), 2003, 22(1): 41-44 |
| 作者姓名： | 陈祥贵 芮光伟 任川洪 王瑞淑 王志民 冉新泽 |
| 作者单位： | 1. 四川工业学院生物工程系,四川,成都,610039 2. 四川大学华西公共卫生学院,四川,成都,610041 3. 第三军医大学预防医学系,重庆,400038 |
| 基金项目： | 四川省科委应用基础研究重点项目 (99C0 72 ) |
| 摘 要： | 大鼠肠上皮细胞株IEC 6具有正常的未分化的肠上皮隐窝细胞的特征 ,在营养学研究中具有重要应用价值。本研究采用相差显微镜、透射电镜、扫描电镜等技术观察细胞形态结构 ,采用MTT法研究血清和血清替代品对ICE 6细胞生长的影响。结果表明 :引进的IEC 6细胞具有典型的正常肠上皮隐窝细胞的形态特征、超微结构和生长特性 ,表明的细胞株未发生转化或变异。不同的血清和血清替代品对细胞增殖的效应有差异 ,2 %的透析胎牛血清能维持细胞良好的增殖状态 ,可用于研究正常培养条件下外源核苷酸对肠上皮细胞的作用。 |
| 关 键 词： | IEC-6 细胞培养 增殖 透析血清 |
| 文章编号： | 1000-5722(2003)01-0041-04 |
| 修稿时间： | 2002-04-13 |
Culture,Identification and Growth characteristics of Cell IEC-6 |
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| CHEN Xiang gui ,RUI Guang wei ,REN Chuan hong ,WANG Rui shu ,WANG Zhi min ,RAN Xin ze. Culture,Identification and Growth characteristics of Cell IEC-6[J]. Journal of Xihua University(Natural Science Edition), 2003, 22(1): 41-44 | |
| Authors: | CHEN Xiang gui RUI Guang wei REN Chuan hong WANG Rui shu WANG Zhi min RAN Xin ze |
| Affiliation: | CHEN Xiang gui 1,RUI Guang wei 1,REN Chuan hong 1,WANG Rui shu 2,WANG Zhi min 1,RAN Xin ze 3 |
| Abstract: | IEC 6,a normal cell line originally derived from the rat small intestinal crypt,was valuable in nutritional investigations.In this study,phase contrast microscope,transmission and scan electron microscope were applied to observe the form and constructure;MTT was used to investigate the effects of sera or sera replacement on proliferiation of IEC 6.The results showed that IEC 6 exhibited typical characterastics of normal,undifferent intestinal crypt cell by morbhologic,ultrastructural and growth criterion.These Results suggested IEC 6 did not transform of mutate.Various sera and sera relacement had diferent effects on cell proliferation.2% dialyzed fetal sera could maintain IEC 6 active growth and was suitable for the study of effects of exogenous mucleotides on the IEC 6 cell.in normal culture conditions. |
| Keywords: | IEC-6 cell culture proliferation dialyzed fetal sera |
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