| 免疫比浊法定量检测葡聚糖的研究 |
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| 引用本文： | 柳颖,蚁细苗,林荣珍,曾练强,黄曾慰,马步,梁达奉. 免疫比浊法定量检测葡聚糖的研究[J]. 甘蔗糖业, 2013, 0(5): 52-55 |
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| 作者姓名： | 柳颖 蚁细苗 林荣珍 曾练强 黄曾慰 马步 梁达奉 |
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| 作者单位： | [1]广州甘蔗糖业研究所广东省甘蔗改良与生物炼制重点实验室,广东广州510316 [2]广西糖业研发中心,广西南宁530002 |
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| 基金项目： | 现代农业产业技术体系建设专项、八桂学者建设工程专项经费资助 |
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| 摘 要： | 葡聚糖影响甘蔗品质及制糖生产，主要表现在糖分损失、糖液粘度增加、过滤困难、结晶异常、糖产品适用性受限等，从而降低提糖率，增加制炼成本，影响糖产品质量。本文对单克隆抗体比浊法测定葡聚糖的方法学进行研究，结果表明，该方法能快速准确测定糖厂蔗汁等在制品及产品中葡聚糖含量，线性范围10～500 mg/L，检测限为10 mg/L，加标回收率98%～102%，RSD〈4%，灵敏度1.3 mg/L。
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| 关 键 词： | 葡聚糖 甘蔗品质 制糖 免疫比浊 |
The Immunoturbidimetry Methods for Quantitative Detection of Dextran |
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| LIU Ying,YI Xi-miao,LIN Rong-zhen,ZENG Lian-qiang,HUANG Zeng-wei,MA Bu,LIANG Da-feng. The Immunoturbidimetry Methods for Quantitative Detection of Dextran[J]. Sugarcane and Canesugar, 2013, 0(5): 52-55 |
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| Authors: | LIU Ying YI Xi-miao LIN Rong-zhen ZENG Lian-qiang HUANG Zeng-wei MA Bu LIANG Da-feng |
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| Affiliation: | 1,2 (1Guangzhou Sugarcane Industry Research Institute/Guangdang Key Lab of Sugarcane Improvement ＆ Biorefinery, Guangzhou 510316; 2Guangxi Sugarcane Industry R＆D Center, Nanning 530002) |
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| Abstract: | Dextran causes marked harmful impact on sugarcane quality and sugar production, which leads to severe sugar loss, increased viscosity of the flow, elongated crystals, expensive yield loss and declined quality of sugar. Based on the newly-generated monoclonal antibody, a reliable immunoturbidimetric method for quantitative detection of dextran was developed. This article studied the proformance of the method, which was proved to be a rapid, correct and convenient tool for detecting dextran in raw material, by-products and end-products of sugar. Results showed that, the linearity was good in the range of 0.01~0.5 mg/mL; the sensitivity was 1.3 mg/L; RSD was less than 4%; the minimum detectability was 0.01 mg/mL; the standard addition recovery rate of the sugar samples were between 98%~102%. |
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| Keywords: | Dextran Sugarcane quality Sugar production Immunoturbidimetry |
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