镰刀菌Fusariumsp sp. JN 158色素的鉴定及其抗肿瘤活性

通过对镰刀菌发酵所产色素的分离及结构的分析,筛选其抗肿瘤的活性成分。采用形态与分子生物学方法对镰刀菌株鉴定,利用HPLC的对该菌株发酵所产的色素进行分离,分离的产物用紫外、红外、核磁进行结构鉴定,使用MTT的方法筛选抗肿瘤的活性成分。结果表明:该镰刀菌所产色素共6个不同的峰,相对分子质量均在380左右,其中VI号峰结构新颖,为花青苷类色素,分子式为C17H18O10,能抑制A539、MCF-7、MKN-45、Hep G2、SW620五种不同肿瘤细胞的增殖,并呈现浓度的依赖性,其中MCF-7乳腺癌细胞抑制最明显(P0.01),故VI号峰色素可能是一种很有前景的防治乳腺癌的化合物。

Pigment Production by Fermentation of Fusarium(Fusarium sp. JN158) and It's Anti-Cancer Activity

Through the separation and structural analysis of the pigment produced by Fusarium fermentation,the research aims to screen the anti-tumor active components. Fusarium was identified by using morphological and molecular biology methods. The pigmen was isolated by HPLC,the production structure was identified by UV,IR,NMR.,and the screening of anti-tumor activity was by MTT. The results showed that the pigment from Fusarium produced a total of six different peaks,the molecular weight is about 380,the VI peak,which is the anthocyanins,can inhibit the A539,MCF-7,MKN-45,HepG2,SW620 five different tumor cell proliferation in a concentration- dependent manner,among them MCF-7 breast cancer cells were inhibited most (P<0.01),so the VI compound with a new structure from Fusarium sp JN 158,may be a good treatment for breast cancer.