| 人体肠道菌群对大豆皂苷Ⅱ的体外代谢转化 |
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| 引用本文： | 桑尚源,光翠娥,江波. 人体肠道菌群对大豆皂苷Ⅱ的体外代谢转化[J]. 食品与生物技术学报, 2015, 34(5): 470-474 |
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| 作者姓名： | 桑尚源 光翠娥 江波 |
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| 作者单位： | 食品科学与技术国家重点实验室，江南大学，江苏 无锡 214122,食品科学与技术国家重点实验室，江南大学，江苏 无锡 214122,食品科学与技术国家重点实验室，江南大学，江苏 无锡 214122 |
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| 摘 要： | 为考察人体肠道菌群对大豆皂苷Ⅱ的体外代谢转化作用,在牛脑牛心培养液(BHI)和磷酸盐缓冲液(PBS)两种离体模型下,研究了大豆皂苷Ⅱ在48 h内被肠道菌群代谢转化过程,并用HPLC/MS系统对其转化产物进行分析鉴定。结果显示,大豆皂苷Ⅱ先被菌群酶转化为大豆皂苷Ⅳ,在48 h被最终转化为大豆皂苷元B,表明大豆皂苷在肠道微生物作用下,其糖基被逐步水解生成相对分子质量更小、疏水性更强的代谢物。
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| 关 键 词： | 大豆皂苷Ⅱ；大豆皂苷元B；肠道菌群；高效液相；质谱 |
In vitro Metabolism of Soyasaponin II by Human Intestinal Microflora |
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| SANG Shangyuan,GUANG Cuie and JIANG Bo. In vitro Metabolism of Soyasaponin II by Human Intestinal Microflora[J]. Journal of Food Science and Biotechnology, 2015, 34(5): 470-474 |
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| Authors: | SANG Shangyuan GUANG Cuie JIANG Bo |
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| Affiliation: | State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China,State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China and State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China |
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| Abstract: | To investigate the metabolism of soyasaponin II by human intestinal microorganisms,the in vitro metabolic transformation of soyasaponin II by intestinal flora in the Brain Heart Infusion(BHI)broth and Phosphate Buffered Saline(PBS)systems was studied for 48 hours,and its metabolites were analyzed and identified by HPLC/MS. The results showed that soyasaponin II was firstly metabolized into soyasaponin IV by intestinal flora and was eventually translated into soyasapogenol B at 48 hours. The above results suggested that soyasaponins lost their sugar moieties due to hydrolysis and yielded smaller and more hydrophobic metabolites by human gut microorganisms. |
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| Keywords: | soyasaponin II soyasapogenol B intestinal microflora HPLC MS |
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