2012—2013年供澳门水产品中致病性弧菌污染状况调查

目的了解供澳门水产品中致病性弧菌的污染状况和三间分布。方法 2012年8月—2013年7月,采用随机抽样的方法收集珠海、中山两地的供澳水产品样品1 510份,利用基于HAND系统多重PCR方法初筛后采用常规分离培养和生化鉴定验证,分析其污染状况,季节性、地域性以及水产品种类的分布。结果供澳水产品样品中弧菌阳性率为59.5%(898/1 510),复合感染率为37.3%(563/1 510)。一年中的5~11月份致病性弧菌总阳性率较高,1月和12月阳性率较低,差异有统计学意义(P0.05)。珠海地区致病性弧菌总阳性率(69.5%)高于中山(51.9%),差异有统计学意义(P0.05)。贝类的致病性弧菌总阳性率(70.6%)高于鱼类(54.4%),差异有统计学意义(P0.05)。结论供澳门水产品中致病性弧菌污染现象严重,需加大致病性弧菌的监测力度,以防致病性弧菌的感染和流行。

Contamination status of pathogenic Vibrios in aquatic products exporting to Macao from 2012 to 2013

To find out the contamination status and distribution of pathogenic Vibrios in aquatic products exporting to Macao.Methods A random sampling method were used to collect samples from Zhuhai and Zhongshan. Samples were preliminary screened by multiplex PCR based on HAND system and confirmed by traditional culture and biochemical identification. The contamination status and seasonal, regional and species distribution were finally analyzed. Results Compared with 37.3% coexistent infection rate, the positive rate of Vibrios in aquatic samples was 59.5%. The positive rate of pathogenic Vibrios from May to November was significantly higher than that of January and December. The overall positive rate in Zhuhai district was 69.5%, higher than that in Zhongshan (51.9%) and the difference was statistically significant (P<0.05). The overall positive rate of shellfish was 70.6%, higher than that of fish (54.4%) and the difference was statistically significant (P<0.05).Conclusion The contamination status of pathogenic Vibrios was serious in aquatic products exporting to Macao and needed to monitor to prevent infection.