石家庄市131株食源性蜡样芽胞杆菌毒力基因的分布

目的研究石家庄市食源性蜡样芽胞杆菌毒力基因的分布及毒力活性,了解蜡样芽胞杆菌的潜在威胁。方法采用PCR方法,对食品风险监测中分离到的131株蜡样芽胞杆菌进行肠毒素、呕吐毒素9种毒力基因扩增检测,用血平板检测的方法分析蜡样芽胞杆菌的毒力。结果毒力基因携带率较高,至少携带一个毒力基因的菌株达到检出菌总数的99.2%(130/131),溶血素BL基因(hbl ACD)和肠毒素FM基因(ent FM)是石家庄市食源性蜡样芽胞杆菌的主要毒力基因;检出的蜡样芽胞杆菌均产生溶血素BL,检出率为100%。结论腹泻型肠毒素在食品中的分布比较广泛,检出的蜡样芽胞杆菌均具有溶血素,对进食者存在潜在的危险性,今后应加强监控蜡样芽胞杆菌的污染,预防和控制蜡样芽胞杆菌食源性疾病的发生。

Distribution of virulence genes and virulence evaluation of food-borne Bacillus cereus in Shijiazhuang City

To study the distribution of virulence genes, evaluate the virulence of food-borne Bacillus cereus in Shijiazhuang City, and to investigate the potential risk.Methods 9 virulence genes (such as enterotoxin and voitoxin) were detected with PCR method in 131 Bacillus cereus collected from food risk monitoring program. The virulence of pathogens were also analyzed using blood agar plates. Results The carrying rate of virulence genes was high. 99.2%(130/131) of the strains carried at least one virulence gene. hblACD and entFM were the major virulence genes of Bacillus cereus in Shijiazhuang City. All strains produced hemolysin BL.Conclusion Diarrhea enterotoxin was widespread in food, and hemolysin was detected in all pathogens, which caused potential public health risk. More effective monitoring strategies should be developed to prevent and control the occurrence of Bacillus cereus food-borne diseases.