原油生物标志物反吹切割微流控色谱分析

研究了一种反吹切割微流控装置气质联用技术直接分析原油中的正构烷烃类、萜烷类、甾烷类生物标志物的新分析方法。利用该方法测定了原油中23个正构烷烃、20个萜烷和11个甾烷，保留时间的相对标准偏差均小于0.02%，火焰离子化检测器测定正构烷烃的峰面积的相对标准偏差为3.1%~6.0%。该方法能同时提供保留时间一致的质谱检测信号和火焰离子化检测信号，为原油生物标志物的分析测定提供一种快速、高效的方法。

An ANALYSIS OF BIOMARKERS IN CRUDE OIL BY BACKFLUSH CUTTING AND MICROFLUIDIC CHROMATOGRAPHY

A novel method was proposed for the direct determination of biomarkers, such as n-alkanes, terpanes and steranes in crude oil by GC/MS coupling with backflush cutting technique and micro-fluidic splitter. The variability of 23 n-alkanes, 20 terpanes and 11 steranes was examined and the relative standard deviation (RSD) of retention time were less than 0.02%, while the RSD of peak area of n-alkanes by flame ion detector were in the range of 3.1~6.0%. This method can simultaneously detect mass and flame ion signals with the same retention time, it provides a fast and effective way for the determination of biomarkers in crude oils.