Parallel and high sensitive photonic crystal cavity assisted read-out for DNA-chips |
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| Authors: | Ferruccio Pisanello Luigi Martiradonna Pier Paolo Pompa Tiziana Stomeo Antonio Qualtieri Giuseppe Vecchio Stefania Sabella Massimo De Vittorio |
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| Affiliation: | 1. National Nanotechnology Laboratory of CNR/INFM, ISUFI – Università del Salento, via per Arnesano, Lecce 73100, Italy;2. Laboratoire Kastler Brossel, CNRS UMR8552, Universitè Pierre et Marie Curie, Ecole Normale Supérieure, 4 place Jussieu, 75252 Paris Cedex 05, France;3. Italian Institute of Technology (IIT), Center for Bio-Molecular Nanotechnology, Via Barsanti, 1 – 73010 Arnesano, Lecce, Italy;1. Resident, Stomatology Special Consultation Clinic, Department of Prosthodontics, Ninth People''s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China;2. Resident, Stomatology Special Consultation Clinic, Department of Prosthodontics, Ninth People''s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China;3. Resident, Stomatology Special Consultation Clinic, Department of Prosthodontics, Ninth People''s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China;4. Professor, Department of Orthodontics, Ninth People''s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China;6. Professor, Stomatology Special Consultation Clinic, Department of Prosthodontics, Ninth People''s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China;1. Facultad de Ciencias, Universidad Autónoma del Estado de México, Campus El Cerrillo, Piedras Blancas, Carretera Toluca - Ixtlahuaca Kilómetro 15.5, Edo. de México C.P. 50200, México;2. Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colon esquina con Paseo Tollocan s/n, Toluca C.P. 50120, México;3. Laboratorio de Fotomedicina, Biofotónica y Espectroscopía Láser de Pulsos Ultracortos, Facultad de Medicina, Universidad Autónoma del Estado de México, Jesús Carranza y Paseo Tollocan s/n, Toluca C.P. 50120, México;4. Laboratorio de Investigación y Desarrollo de Materiales Avanzados, Facultad de Química, Universidad Autónoma del Estado de México, Campus Rosedal, Km 14.5 Carretera Toluca-Atlacomulco, San Cayetano de Morelos, Toluca C.P. 50925, México |
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| Abstract: | In this work we propose a new technological approach aimed at improving the performances of DNA-chips in terms of detection sensitivity, signal-to-noise ratio and parallel analyses (spatial and spectral). It is based on the efficient enhancement of markers fluorescence through the insertion of photonic crystal nanocavities (PhC) in DNA-chips, thus giving higher sensitivity and allowing detection of small amounts of target biomolecules in the investigated solution. Moreover, this strategy univocally associates a specific emission wavelength to a specific nanocavity (and to the bio-probe immobilized on it), therefore allowing to infer the presence of a determined element in the solution by a simple spectral analysis of the optical response of the read-out region. This guarantees parallel detection of multiple elements and faster analysis time. The proposed 2D-PhC cavity assisted bio-chip read-out can be easily extended from DNA to a wide range of biomolecules, such as proteins, antibodies, aptamers, receptors. |
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