Channel height dependent protein nucleation and crystal growth in microfluidic devices |
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| Authors: | Malika Lounaci Yong Chen Pascal Rigolet |
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| Affiliation: | 1. Ecole Normale Supérieure of Paris, CNRS-ENS-UPMC UMR 8640, 24 rue Lhomond, 75231 Paris, France;2. Institute for Integrated Cell-Material Sciences, Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, 606-8507 Kyoto, Japan;3. Laboratoire de Dynamique des Microtubules en Physiopathologie, Université Paris-Sud 11 Faculté de Pharmacie, 5 rue Jean-Baptiste Clément, Châtenay-Malabry 92296, France;1. Department of Chemical and Biomolecular Engineering, University of Houston, 4726 Calhoun Road, Houston, TX 77204, USA;2. Department of Chemistry, University of Houston, 3585 Calhoun Road, Houston, TX 77204, USA;1. Particulate Fluids Processing Centre, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia;2. MSACT Research and Consulting, Exeter EX2 8GP, United Kingdom;3. School of Chemistry and Bio21 Institute, The University of Melbourne, Parkville, Victoria 3010, Australia;1. School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran;2. Hematology Department, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran;3. Chemical Engineering Department, Faculty of Engineering, Tarbiat Modares University, Tehran, Iran;4. Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran;5. Department of Toxicology & Pharmacology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran;1. Institute of Photonics and Bio-medicine (IPBM), School of Science, East China University of Science and Technology (ECUST), No. 130, Meilong Road, Shanghai, 200237, China;2. Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan;3. Department of Applied Physics, Graduate School of Science, Osaka University, Yamadaoka, Suita-city, Osaka 565-0871, Japan |
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| Abstract: | We report on results of a study of protein crystallization in microfluidic devices with different channel heights. Multilayer soft lithography has been used for the fabrication of devices with integrated micro-valves and crystallization channels of height in the range from 15 μm to 180 μm. To demonstrate the channel height dependent nucleation and crystal growth, a standard batch crystallization solution composed of 60 mg/ml lysozyme, 100 mM acetate buffer pH 4.6 and 1.5 M NaCl was used with minimized sample quantity. Our results show that deep channels favorite the nucleation whereas shallow ones favorite the crystal growth. When the channel height is less than 50 μm the number of lysozyme crystals is dramatically reduced whereas their mean size is increased. Furthermore, our results also show the feasibility of decoupling nucleation and crystal growth in a stair-like channel which should facilitate the appearance of single crystals suitable for X-ray diffraction. |
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