Bi-Functional Radiotheranostics of 188Re-Liposome-Fcy-hEGF for Radio- and Chemo-Therapy of EGFR-Overexpressing Cancer Cells |
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Authors: | Yi-Shu Huang Wei-Chuan Hsu Chien-Hong Lin Sheng-Nan Lo Chu-Nian Cheng Ming-Syuan Lin Te-Wei Lee Chih-Hsien Chang Keng-Li Lan |
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Affiliation: | 1.Division of Isotope Application, Institute of Nuclear Energy Research, Taoyuan 32546, Taiwan; (Y.-S.H.); (W.-C.H.); (C.-H.L.); (S.-N.L.); (C.-N.C.); (M.-S.L.); (T.-W.L.);2.Department of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan;3.Department of Oncology, Taipei Veterans General Hospital, Taipei 112, Taiwan;4.Institute of Traditional Medicine, School of Medicine, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan |
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Abstract: | Epidermal growth factor receptor (EGFR) specific therapeutics is of great importance in cancer treatment. Fcy-hEGF fusion protein, composed of yeast cytosine deaminase (Fcy) and human EGF (hEGF), is capable of binding to EGFR and enzymatically convert 5-fluorocytosine (5-FC) to 1000-fold toxic 5-fluorocuracil (5-FU), thereby inhibiting the growth of EGFR-expressing tumor cells. To develop EGFR-specific therapy, 188Re-liposome-Fcy-hEGF was constructed by insertion of Fcy-hEGF fusion protein onto the surface of liposomes encapsulating of 188Re. Western blotting, MALDI-TOF, column size exclusion and flow cytometry were used to confirm the conjugation and bio-activity of 188Re-liposome-Fcy-hEGF. Cell lines with EGFR expression were subjected to treat with 188Re-liposome-Fcy-hEGF/5-FC in the presence of 5-FC. The 188Re-liposome-Fcy-hEGF/5-FC revealed a better cytotoxic effect for cancer cells than the treatment of liposome-Fcy-hEGF/5-FC or 188Re-liposome-Fcy-hEGF alone. The therapeutics has radio- and chemo-toxicity simultaneously and specifically target to EGFR-expression tumor cells, thereby achieving synergistic anticancer activity. |
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Keywords: | cytosine deaminase epidermal growth factor receptor 5-fluorocuracil liposome prodrug rhenium-188 |
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