胶体金免疫层析法测定牛羊肉中掺杂鸡肉

目的 建立胶体金免疫层析法快速检测牛羊肉中掺杂鸡肉的方法.方法 采用柠檬酸三钠制备胶体金溶液,以真空干燥的方式制备有金标抗体的微孔试剂,将鸡IgY包被于硝酸纤维素膜(NC膜)的检测线(T线),兔抗羊抗体包被于硝酸纤维素膜(NC膜)的质控线(C线),向硝酸纤维素膜依次贴上吸水纸和样品垫,切条组装成试纸条.样品经磷酸盐处理...

Determination of chickenadulteration in beef and mutton by colloidal gold immunochromatography

A colloidal gold immunochromatographic method for the detection of chicken adulteration in beef and mutton was established. Based on the competitive inhibition model, the specific antibody IgY of poultry was used as the target to combine with colloidal gold. Colloidal gold solution was prepared by trisodium citrate, and microporous reagent with gold labeled antibody was prepared by vacuum drying. Chicken IgY was conjugated on the detection line (T) of nitrocellulose (NC) membrane, and Rabbit anti sheep antibody was conjugated on the quality control line (C) of nitrocellulose (NC) membrane. Attach absorbent paper and sample pad to nitrocellulose membrane in turn, cut and assemble them into test strips. The sample was treated with phosphate, added into the micropores of gold labeled antibody then detected by test strips. The color signal of T / C line was detected by colloidal gold analyzer. The results showed that the detection limit of this method was 3%, with good repeatability and specificity. The other animal tissues did not interfere with it, and the detection time was only 15 minutes. The method is simple, sensitive and specific, and can be used for on-site rapid screening and detection of chicken adulteration in beef and mutton.