罗伊氏菌素与儿茶素协同对变异链球菌的抑制作用

为探讨儿茶素与罗伊氏菌素(主要抗菌成分为3-羟基丙醛(3-hydroxypropionaldehyde,3-HPA))对诱发龋齿的变异链球菌的抑制作用及其机制,本研究测定了罗伊氏菌素与儿茶素单独使用和联合作用时对变异链球菌的最小抑菌浓度(minimum inhibitory concentration,MIC),经计算...

Synergistic Inhibition Mechanism of Reuterin and Catechin against Streptococcus mutans

In order to investigate the inhibitory effect of catechin and reuterin(3-hydroxypropionaldehyde(3-HPA))on the caries pathogen Streptococcus mutans and to dissect the underlying mechanisms,the minimum inhibitory concentrations of reuterin and catechin alone and in combination were determined in the present study.By calculating the fractional inhibitory concentration index(FICI),it was found that catechin(1/8 MIC)and 3-HPA(1/4 MIC)had a synergistic effect when used together.The combination of the two drug resulted in a significant reduction in the contents of biofilm,extracellular polysaccharide(EPS)and organic acid(P<0.05),more effective than either drug alone.Scanning electron microscopic results showed that the biofilm structure in the combination treatment group significantly changed compared to either individual treatment group,accompanied by a decrease in biofilm thickness and the number of bacterial cells.Collectively,the above results demonstrated that the combination treatment of catechin and 3-HPA could inhibit bacterial aggregation and adhesion,impede bacterial cells from being involved in substrate metabolism,increase acidity and delay biofilm growth and maturation,and thus could be used as a novel antibacterial agent against caries.This study will provide a theoretical basis for the development of natural and safe anti-caries products.