离子色谱法测定海带中甘露醇的含量

目的 建立测定海带中甘露醇含量的离子色谱法.方法 通过对色谱柱、流动相等色谱条件优化,考察对海带中甘露醇及其他组分的分离效果.结果 采用DIONEX CarboPac PA10(4 mm×250 mm)色谱柱,以H2O:NaOH溶液(c=250 mmol/L)(90:10,V:V)为流动相,流速为0.80 mL/min...

Determination of mannitol in Laminaria japonica by ion chromatography

Objective To establish a method for the determination of mannitol in Laminaria japonica by ion chromatographic. Methods The separation effect of mannitol and other components in Laminaria japonica kelp were investigated by optimizing the chromatographic conditions of column and flow equivalence. Results DIONEX CarboPac PA10 (4 mm×250 mm) column was used. H2O:NaOH solution (c=250 mmol/L) (90:10, V:V) was used as the mobile phase, and the flow rate was 0.80 mL/min. Under these conditions, mannitol showed a good linear relationship in the range of 1.01-80.83 g/mL. The correlation coefficient was 0.9926, the minimum limit of detection was 0.03 g/mL, the minimum limit of quantification was 0.10 g/mL, and the average recovery was 101.61%. Conclusion The method is a simple, sensitive, accurate and effective method for detection of mannitol in Laminaria japonica.