响应面法优化淀粉葡糖苷酶的酶反应体系

为建立并优化淀粉葡糖苷酶的酶反应体系,通过单因素分析和响应面实验设计,以淀粉葡糖苷酶能够直接作用的天然底物即淀粉为作用对象,以反应速度为指标,考察可溶性淀粉浓度、淀粉葡糖苷酶浓度、反应时间3 个因素对酶促反应速度的影响.结果表明,所建立淀粉葡糖苷酶的最佳酶反应体系为可溶性淀粉质量浓度10.30 mg/mL、淀粉葡糖苷酶...

Optimization of Enzyme Reaction System for Amyloglucosidaseby Response Surface Methodology

The optimal enzyme reaction systems of amyloglucosidase was established, in a physiological simulation condition of human body, by using starch as natural substrate by single-factor optimization and Box-Behnken design experiment with soluble starch concentration, amyloglucosidase concentration and reaction time as investigated reaction factors to explore the reaction rate of amyloglucosidase on substance. The results showed that the enzyme reaction rate of amyloglucosidase reached (15.17±1.84) mmol/(L·min) under its optimal enzyme reaction system with 10.30 mg/mL soluble starch, 1.15 U/mL amyloglucosidase, and 7.00 min reaction time, whose relative error with theoretical predictions in the obtained model is 1.69%. The predicted values of the enzymatic reaction rates of the above models were highly consistent with the actual results, indicating that the established reaction system could predict the actual enzyme activity commendably. Hence the system of amyloglucosidase by response surface methodology is reliable and can be used effectively in related researches such as screening out the efficient glycosidase and obtaining more reliable data.