黑小麦麸皮抗氧化活性成分提取及其抗氧化能力评价

黑小麦麸皮是黑小麦加工过程中的副产物，其富含多种生理活性物质，包括花青素、酚酸类物质、膳食纤维等。以黑小麦麸皮为原料，利用不同溶剂对其抗氧化活性成分进行提取并对其抗氧化能力评价研究。结果表明，75%乙醇提取物干物质得率最高，为10.72%；50%乙醇提取总酚含量最高，为2.9 mg/100 mL；50%乙醇提取物对1,1-二苯基-2-三硝基苯肼（DPPH）清除力最强，75%乙醇提取物对DPPH抗氧化物提取能力最强；75%丙酮提取物对2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）清除力最强，75%甲醇提取物对ABTS抗氧化物提取能力最强。综合比较得出，50%乙醇更适于黑小麦麸皮抗氧化活性成分提取。

Extraction of Antioxidant Effective Components from Black Wheat Bran and Evaluation of its Antioxidant Capacity

Black wheat bran is by-products of the black wheat processing, which is rich in many physiological active substances, including anthocyanins, phenolic acids and dietary fiber. In this study, the antioxidant activity of black wheat bran was extracted with different solvents and its antioxidant capacity was evaluated. The results showed that the dry matter yield of 75% ethanol extract was the highest, which was 10.72%. The content of total phenol extracted by 50% ethanol extract was the highest, which was 2.9 mg/ 100 mL. The scavenging power of DPPH was the strongest in 50% ethanol extract, and the antioxidant extraction capacity of DPPH was the strongest in 75% ethanol extract. 75% acetone extract had the strongest scavenging effect on ABTS, and 75% methanol extract had the strongest antioxidant extraction effect on ABTS. Therefore, the antioxidant capacities of different solvent extracts are different, and the comprehensive comparison shows that 50% ethanol is more suitable for the extraction of antioxidant active components from black wheat bran.