液相色谱-质谱联用测定鸡蛋中4-乙酰氨基苯甲酸的残留

目的 建立液相色谱-质谱法检测鸡蛋中4-乙酰氨基苯甲酸残留的方法.方法 样品经乙腈提取,通过式吸附净化和阴离子交换固相萃取净化,液相色谱分离,四极杆-静电场轨道阱质谱定性定量测定,平行反应监测模式检测,外标法定量.结果 4-乙酰氨基苯甲酸保留时间为5.6 min,在2.5～50μg/kg范围内呈现良好的线性关系,日内、...

Residual determination of p-acetylamino-benzoic acid in eggs by liquid chromatography-mass spectrometry

Objective To establish a method for the determination of p-acetaminobenzoic acid residues in eggs by liquid chromatography-mass spectrometry. Methods The samples were extracted by acetonitrile, purified by pass-through cleanup and solid phase extraction with anion exchange, separated by liquid chromatography, qualitatively and quantitatively determined by quadrupole-orbitrap mass spectrometry, detected by parallel reaction monitoring mode, and quantified by external standard method. Results The retention time of p-acetylamino-benzoic acid was 5.6 min, and showed a good linear relationship in the range of 2.5-50 μg/kg. The precisions of intra-day and inter-day were less than 15%. The recoveries were 70%-80% and the limit of quantitation was 2.5 μg/kg. Conclusion This method is fast, accurate and highly sensitive, which provides a fast and accurate technical means for monitoring the illegal use of isopropyl inosine and monitoring the residues in eggs.