3种方法鉴定鸡肉粉中沙门氏菌的比对研究

目的采用3种方法对鸡肉粉中沙门氏菌进行检测。方法3份样品的前处理依据组织方提供的《作业指导书》进行,每瓶样品直接加入4.5 mL灭菌去离子水复溶,作为初始样本,后续实验依据GB4789.4-2016《食品安全国家标准食品微生物学检验沙门氏菌检验》操作。鉴定分离出疑似菌后,加入了实时荧光定量PCR法、基质辅助激光解吸电离飞行时间质谱法2种方法作为辅助检测,并用全自动微生物鉴定系统进行生化鉴定,再结合生化鉴定与血清学实验综合评判检测结果。结果使用3种鉴定方法在JS015、JS110、JS140样本中均检出沙门氏菌。结论本研究采用的实时荧光定量PCR法、基质辅助激光解吸电离飞行时间质谱法2种方法均能快速准确鉴定出沙门菌,特别是基质辅助激光解吸电离飞行时间质谱法和血清学分型配合使用,对沙门菌的快速鉴定有重要意义。

Comparative study on the identification of Salmonella in chicken powder by 3 methods

Objective Salmonella was detected in three portions of chicken powder by several methods. Methods The pretreatment of the three samples shall be conducted in accordance with the Work Instruction provided by the organization. Each bottle of samples shall be directly added into 4.5mL sterilized deionized water for re-dissolution as the initial samples, and the subsequent experiments shall be conducted in accordance with GB 4789.4-2016 National Food Safety Standard Food Microbiology Test for. After identification of the suspected bacteria, Quantitative Real-time PCR and MALDI-TOF-MS were added as the auxiliary detection methods. Biochemical identification was carried out with the automatic microbial identification system, and the detection results were comprehensively evaluated with the combination of biochemical identification and serological experiment. Results Salmonella was detected in samples JS015, JS110 and JS140 by three identification methods. Conclusion The two methods adopted in this study, Quantitative Real-time PCR and MALDI-TOF-MS, were able to identify Salmonella quickly and accurately. In particular, MALDI-TOF-MS method and serum credit type were used in combination, which was of great significance for the rapid identifi-cation of Salmonella.