高效液相色谱法测定保健食品中非法添加盐酸二氧丙嗪的不确定度分析

目的采用高效液相色谱法测定保健食品中非法添加盐酸二氧丙嗪的含量,进行不确定度分析。方法依据标准JJF 1059.1-2012《测量不确定度评定与表示》,建立数学模型,对测定不确定度的来源进行分析和量化。结果保健食品中非法添加盐酸二氧丙嗪含量为1.90 g/kg时,其相对扩展不确定度为0.088,测定结果可表示为X=(1.90±0.17)g/kg(k=2)。根据测量结果分析,在影响检测结果不确定度的各分量中,拟合标准曲线求溶液中目标物浓度所引入的不确定度是对结果影响较大的不确定度分量,可通过减少这个不确定度分量提高测量结果的质量。结论该研究可为实验室评定保健食品中非法添加盐酸二氧丙嗪的测定结果质量提供参考。

Uncertainty evaluation for determination of dioxopromethazine hydrochloride in health food by high performance liquid chromatography

Objective To evaluate the uncertainty of dioxopromethazine hydrochloride content illegally added in health food by high performance liquid chromatography.Methods According to the standard JJF 1059.1—2012 Evaluation and expression of uncertainty in measurement,the mathematical model was established to analyze and quantify the sources of measurement uncertainty.Results The content of dioxopromethazine hydrochloride in health food was 1.90 g/kg,the relative expanded uncertainty was 0.088,and the determination result was expressed as X=(1.90±0.17)g/kg(k=2).According to the analysis of the measurement results,the main factor coming from contents of targeted object lines fitting influenced the detection results mostly.Through reducing this factor,the quality of the determination results could be increased.Conclusion This study can provide a reference for laboratory to evaluate the quality of the determination results of illegally added dioxopromethazine hydrochloride in health food.