高效液相色谱法测定灭菌乳中糠氨酸的不确定度评定

目的 评定高效液相色谱法测定灭菌乳中糠氨酸含量结果的不确定度.方法 依据NY/T939-2016《巴氏杀菌乳和UHT灭菌乳中复原乳的鉴定》测定灭菌乳中糠氨酸的含量,建立高效液相色谱法测定灭菌乳中糠氨酸含量的不确定度评定的数学模型,全面分析实验过程中各不确定度的来源,并对各不确定度进行分量量化及合成,计算灭菌乳中糠氨酸的...

Uncertainty evaluation for the determination of furosine in sterilized milk by high performance liquid chromatography

Objective To evaluate the uncertainty of determination of furosine in sterilized milk by liquid chromatography. Investigate the steps that contribute the most to the uncertainty of the final result, and control the key steps. Methods The content of furosine in sterilized milk was determined according to NY/T939-2016 "Identification of reconstituted milk in pasteurized milk and UHT sterilized milk". The mathematical model of uncertainty evaluation for determination of furosine in sterilized milk by high performance liquid chromatography was established. The sources of uncertainty in the experiment process were analyzed comprehensively, and each uncertainty was quantified and synthesized. The standard uncertainty and expanded uncertainty of synthesis of furosine in sterilized milk were calculated. Results When the content of furosine in sterilized milk was 151.0 mg/100 g protein, the expanded uncertainty of measurement results was 5.8 mg/100 g protein (k=2). Conclusion The evaluation method of medium-range uncertainty in the determination of furosine by liquid chromatography is established. Pretreatment of furosine, protein detection and measurement repeatability are the main sources of measurement errors.