基于网络药理学和文献探讨异喹啉类生物碱抗肝癌研究进展

探索五种异喹啉类生物碱抗肝癌的作用机制。借助TCMSP、String、venny数据库与WebGestalt在线分析软件获取靶点并进行蛋白互作网络（PPI）、基因本体（GO）、基因相互作用（KEGG）功能富集分析，利用Cytoscape软件构建网络。同时查阅文献并进行归纳、整理和分析得到小檗碱型中的小檗碱、双苄基类生物碱的粉防己碱、苯菲啶喹啉类的血根碱、吗啡烷类中的青藤碱和吡咯菲里啶中的石蒜碱等几种常见的生物碱，根据结构作用机制阐述每一类代表性异喹啉类生物碱治疗肝癌的潜力及抗肝癌作用机制。五个活性成分共筛选得到52个作用靶标如p53（抑癌基因）、NCOA2（核受体共激活剂2）、IL-2（白介素2），主要涉及钙离子信号通路、雌激素受体信号通路和PI3K/Akt信号传导途径等通路。通过网络药理学和文献收集证实异喹啉类生物碱可能参与调控PI3K-Akt通路等，可以通过多靶点、多通路对肝癌产生治疗作用，为进一步研究异喹啉类生物碱抗肝癌的临床研究提供参考和依据。

Research Progress of Isoquinoline Alkaloids against Liver Cancer Based on Network Pharmacology and Literature

To explore the anti-hepatocellular mechanism of five kinds of isoquinoline alkaloids, with the help of TCMSP, string, venny database and WebGestalt online analysis software to obtain the target and perform protein interaction network (PPI), gene ontology (GO), gene interaction (KEGG) function enrichment analysis, and use Cytoscape software to construct the network. At the same time, berberine in proberberine, Tetrandrine in dibenzyl alkaloids, sanguinarine in Phenphenanthroline, sinomenine in morphine and lycopine in pyrrolidine were obtained by literature review, induction, sorting and analysis. According to the structural mechanism of action, the potential of each type of representative isoquinoline alkaloid in the treatment of liver cancer and the mechanism of anti-liver cancer were expounded. Five active ingredients were screened and 52 targets such as p53 (tumor suppressor gene), NCOA2 (nuclear receptor co-activator 2), IL-2 (interleukin 2) were obtained which mainly involved calcium ion signaling pathway, estrogen receptor signaling Pathways and PI3K/Akt signaling pathways. Through network pharmacology and literature collection, it is confirmed that isoquinoline alkaloids may be involved in the regulation of PI3K-Akt pathway, etc., and can have therapeutic effects on liver cancer through multiple targets and multiple pathways. This paper can provide reference and basis for further clinical research on the anti-liver cancer effect of isoquinoline alkaloids.