Increase in the γ-linolenic acid content by solvent winterization of fungal oil extracted fromMortierella genus |
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Authors: | T Yokochi M T Usita Y Kamisaka T Nakahara O Suzuki |
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Affiliation: | (1) National Chemical Laboratory for Industry, 1-1, Higashi, Tsukuba, Ibaraki, Japan;(2) National Institute of Science and Technology, National Science and Technology Authority, Manila, Philippines |
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Abstract: | The fungal oil extracted fromMortierella ramanniana var.angulispora (IFO 8187) was solvent winterized in order to raise the content of γ-linolenic acid (GLA). Effects of winterization conditions
(solvent, oil concentration in the solvent and temperature) and changes of glyceride compositions were discussed. The fungal
oil was separated into four diglycerides and 17 triglycerides (TG) with high performance liquid chromatography. The predominant
species were POO, POP and LOP, whose contents were 24.4, 22.9 and 9.4% of the total TG, respectively. Ethanol at 4°C gave
the highest GLA content of 10.5% in spite of lower yield than with acetone at −20°C. The highest separation efficiency for
GLA (ηGLA) was 0.27 with acetone at −20°C and 10% oil concentration, resulting in 8.3% of GLA from the fungal oil at 5.7% LGA. In case
of lower oil concentration at 5–20%, ηGLA showed higher in the following order: acetone (−20°C)>n-hexane (−20°C)>acetone (4°C)>petroleum ether (−20°C). The winterization process also proved to be effective for the separation
of TG type, Sa2U (Sa; saturated fatty acid; U, unsaturated fatty acid) into the crystallized fraction and SaU2 into the liquid fraction. Acetone at −20°C showed higher separation efficiency for triunsaturated TG than the other solvents. |
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Keywords: | Crystallization fungal oil glyceride composition linolenic acid (GLA) Mortierella ramanniana separation efficiency Triglyceride composition winterization |
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