| 海藻酸钠明胶协同固定化黑曲霉脂肪酶 |
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| 引用本文： | 王爱玲,杨江科,黄瑛,闫云君.海藻酸钠明胶协同固定化黑曲霉脂肪酶[J].应用化工,2007,36(4):317-320,324. |
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| 作者姓名： | 王爱玲 杨江科 黄瑛 闫云君 |
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| 作者单位： | 华中科技大学生命科学与技术学院,湖北,武汉,430074 |
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| 基金项目： | 国家高技术研究发展计划(863计划);湖北省科技攻关项目 |
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| 摘 要： | 以海藻酸钠明胶为复合载体,采用包埋法制备固定化黑曲霉脂肪酶,考察了海藻酸钠、明胶浓度等因子对固定化效果的影响,比较固定化酶和游离酶对温度、pH等条件的稳定性。结果表明,制备固定化黑曲霉脂肪酶的最优条件为:海藻酸钠、明胶浓度分别为1.25%和0.5%,CaC l2浓度为10%,给酶量为450 IU/g;固定化酶最适温度为35℃,最适pH为9.0,常见有机溶剂和金属离子对固定化酶的活力影响较小。
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| 关 键 词： | 海藻酸钠 明胶 黑曲霉 脂肪酶 包埋法 |
| 文章编号： | 1671-3206（2007）04-0317-04 |
| 修稿时间： | 2007-01-19 |
Immobilization of lipase from Aspergillus Niger in sodium alginate-gelatin |
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| WANG Ai-ling,YANG Jiang-ke,HUANG Ying,YAN Yun-jun.Immobilization of lipase from Aspergillus Niger in sodium alginate-gelatin[J].Applied chemical industry,2007,36(4):317-320,324. |
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| Authors: | WANG Ai-ling YANG Jiang-ke HUANG Ying YAN Yun-jun |
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| Affiliation: | Huazhong University of Science and Technology, College of Life Science and Technology, Wuhan 430074, China |
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| Abstract: | Lipase from Aspergillus Niger was entrapped by drop-wise addition of an aqueous of sodium alginate-gelatin and was immerged in a hardening solution of CaCl2.Effects of immobilization conditions such as alginate concentration,gelatin concentration,CaCl2 concentration and immobilized enzyme stability against changes of temperature and pH were systematically studied.The results indicated that the optimum operation conditions were: the concentration of sodium alginate,gelatin and CaCl2 were 1.25%,0.5% and 10%,respectively.The optimum temperature and pH were 35 ℃ and 9.0.The effects of organic solvents and metal ions were insignificant on the immobilized enzyme. |
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| Keywords: | sodium alginate gelatin Aspergillus Niger lipase entrapping process |
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