Salvianolic acid B inhibits low‐density lipoprotein oxidation and neointimal hyperplasia in endothelium‐denuded hypercholesterolaemic rabbits |
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| Authors: | Jeng‐Jiann Chiu Ming‐Shi Shiao Chien‐Sung Tsai Shing‐Jong Lin Yuh‐Lien Chen |
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| Affiliation: | 1. Division of Medical Engineering Research, National Health Research Institutes, Miaoli, Taiwan;2. Institute of Biomedical Sciences, Chang‐Gung University, Taoyuan, Taiwan;3. Department of Cardiovascular Surgery, National Defense Medical Center, Taipei, Taiwan;4. Division of Cardiology, Department of Internal Medicine, Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan;5. Institute of Clinical Medicine, National Yang‐Ming University, Taipei, Taiwan;6. Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, Taiwan |
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| Abstract: | BACKGROUND: Atherosclerosis and restenosis are inflammatory responses involving free radicals and lipid peroxidation and may be prevented/cured by antioxidant‐mediated lipid peroxidation inhibition. Salvianolic acid (Sal B), a water‐soluble antioxidant obtained from a Chinese medicinal herb, is believed to have multiple preventive and therapeutic effects against human vascular diseases. In this study the in vitro and in vivo inhibitory effects of Sal B on oxidative stress were determined. RESULTS: In human aortic endothelial cells (HAECs), Sal B reduced oxidative stress, inhibited low‐density lipoprotein (LDL) oxidation and reduced oxidised LDL‐induced cytotoxicity. Sal B inhibited Cu2+‐induced LDL oxidation in vitro (with a potency 16.3 times that of probucol) and attenuated HAEC‐mediated LDL oxidation as well as reactive oxygen species (ROS) production. In cholesterol‐fed New Zealand White rabbits (with probucol as positive control), Sal B intake reduced Cu2+‐induced LDL oxidation, lipid deposition in the thoracic aorta, intimal thickness of the aortic arch and thoracic aorta and neointimal formation in the abdominal aorta. CONCLUSION: The data obtained in this study suggest that Sal B protects HAECs from oxidative injury‐mediated cell death via inhibition of ROS production. The antioxidant activity of Sal B may help explain its efficacy in the treatment of vascular diseases. Copyright © 2010 Society of Chemical Industry |
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| Keywords: | salvianolic acid B oxidised low‐density lipoprotein endothelial cells atherosclerosis restenosis |
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