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Cryo‐scanning electron microscopy and light microscopy for the study of fungi interactions
Authors:F. Sempere  M.P. Santamarina
Affiliation:Fondo de Diversidad Fúngica de la Comunidad Valenciana, Unidad Docente de Ecología y Botánica, Departamento de Ecosistemas Agroforestales, Escuela Técnica Superior del Medio Rural y Enología, Universidad Politécnica de Valencia, Avda, Blasco Iba?ez, 21. 46010‐Valencia, Espa?a
Abstract:The application of the cryo‐scanning electron microscopy and light microscopy for the study of the interactions at different environmental conditions between Penicillium oxalicum and Fusarium verticillioides is described. A dual microculture was developed for the light microscopy analysis of the interaction. The microscope and macroscopic examinations were compared. Analysis of Petri plates revealed that F. verticillioides was a competitor for space and nutrients while P. oxalicum was a mycoparasite under the microscopic observations. Microsc. Res. Tech., 2010. © 2010 Wiley‐Liss, Inc.
Keywords:fungi interactions  microscopic analysis  water activity  Penicillium oxalicum  Fusariumverticillioides
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