A continuous ultrasound‐assisted packed‐bed bioreactor for the lipase‐catalyzed synthesis of caffeic acid phenethyl ester |
| |
| Authors: | Hsiao‐Ching Chen Chia‐Hung Kuo Yawo‐Kuo Twu Jiann‐Hwa Chen Chieh‐Ming J Chang Yung‐Chuan Liu Chwen‐Jen Shieh |
| |
| Affiliation: | 1. Department and Graduate Program of Bioindustry Technology, Dayeh University Chang‐Hua, 515, Taiwan;2. Biotechnology Center, National Chung Hsing University, Taichung, 402, Taiwan;3. Graduate Institute of Molecular Biology, National Chung Hsing University Taichung, 402, Taiwan;4. Department of Chemical Engineering, National Chung Hsing University Taichung, 402, Taiwan |
| |
| Abstract: | BACKGROUND: The focus of this paper is the ultrasound‐assisted synthesis of caffeic acid phenethyl ester (CAPE) from caffeic acid and phenyl ethanol in a continuous packed‐bed bioreactor. Immobilized Novozym® 435 (from Candida antarctica) is used as the catalyst. A three‐level–three‐factor Box–Behnken design and a response surface methodology (RSM) are employed to evaluate the effects of temperature, flow rate, and ultrasonic power on the percentage molar conversion of CAPE. RESULTS: Based on ridge max analysis, it is concluded that the optimum condition for synthesis is reaction temperature 72.66 °C, flow rate 0.046 mL min?1, and ultrasonic power 1.64 W cm?2. The expected molar conversion value is 97.84%. An experiment performed under these optimal conditions resulted in a molar conversion of 92.11 ± 0.75%. The enzyme in the bioreactor was found to be stable for at least 6 days. CONCLUSIONS: The lipase‐catalyzed synthesis of CAPE by an ultrasound‐assisted packed‐bed bioreactor uses mild reaction conditions. Enzymatic synthesis of CAPE is suitable for use in the nutraceutical and food production industries. Copyright © 2011 Society of Chemical Industry |
| |
| Keywords: | lipase packed‐bed reactor phenolic acid response surface methodology ultrasonication |
|
|
