Solanum nigrum L. polyphenolic extract inhibits hepatocarcinoma cell growth by inducing G2/M phase arrest and apoptosis |
| |
| Authors: | Cheng‐Hsun Wu Kuang‐Ping Lan Mon‐Yuan Yang Chau‐Jong Wang |
| |
| Affiliation: | 1. Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, ROC;2. Department of Anatomy, China Medical University, Taichung, Taiwan, ROC;3. Department of Laboratory Medicine, Ci‐Shan Hospital, Department of Health Executive, Yuan, ROC;4. Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan, ROC |
| |
| Abstract: | BACKGROUND: Hepatocellular carcinoma (HCC) is a rapidly progressive cancer with poor prognosis. However, there have been no significant new developments in treating liver cancer. To search for an effective agent against HCC progression, we prepared a polyphenolic extract of Solanum nigrum L. (SNPE), a herbal plant indigenous to Southeast Asia and commonly used in oriental medicine, to evaluate its inhibitive effect on hepatocarcinoma cell growth. The growth inhibition of HepG2 cells in vitro and in vivo was determined in the presence of SNPE. RESULTS: We found 1 µg mL?1 SNPE‐fed mice showed decreased tumor weight and tumor volume by 90%. Notably, 2 µg mL?1 SNPE resulted in almost complete inhibition of tumor weight as well as tumor volume. In line with this notion, SNPE reduced the viability of HepG2 cells in a dose‐dependent manner. HepG2 cells were arrested in the G2/M phase of the cell cycle; meanwhile, the protein levels of cell CDC25A, CDC25B, and CDC25C were clearly reduced. Moreover, sub‐G1 phase accumulation and caspases‐3, 8, and 9 cleavages were induced by SNPE. CONCLUSION: This study shows that SNPE is a potent agent for HCC treatment through targeting G2/M arrest and apoptosis induction, achieving cell growth inhibition. Copyright © 2010 Society of Chemical Industry |
| |
| Keywords: | apoptosis caspases G2/M arrest hepatocellular carcinoma Solanum nigrum L polyphenolic extract |
|
|
