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Empirically determined freezing time for quick-freezing with a liquid-nitrogen-cooled copper block |
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| Authors: | P H W W BAATSEN |
| Abstract: | A method is presented to determine freezing time empirically. The method is based on determining the amount of stretch of a skinned muscle fibre while it is being frozen. Freezing time, as determined with this method, lies in between 0·5 and 1·5 ms. |
| Keywords: | Freezing time temporal resolution quick-freezing liquid-nitrogen-cooled copper block electron microscopy |