A universal fluid cell for the imaging of biological specimens in the atomic force microscope |
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Authors: | Sandor Kasas Ksenja Radotic Giovanni Longo Bashkar Saha Livan Alonso‐Sarduy Giovanni Dietler Charles Roduit |
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Affiliation: | 1. Laboratoire de Physique de la Matière Vivante, , Lausanne, Switzerland;2. Département de Neurobiologie Fondamentale, Université de Lausanne, , Lausanne, Switzerland;3. Institute for Multidisciplinary Research, University of Belgrade, , Beograd, Serbia;4. National Centre for Cell Science, , Pune, 411007 Maharashtra, India;5. Department of Pharmaceutical Science, University of Nebraska Medical Center, College of Pharmacy, , Omaha, 68198 Nebraska |
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Abstract: | Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM imaging. An important component of the set‐up is the imaging chamber, whose design determines the nature of the experiments that can be conducted. Many different imaging chamber designs are available, usually designed to optimize a single parameter, such as the dimensions of the substrate or the volume of fluid that can be used throughout the experiment. In this report, we present a universal fluid cell, which simultaneously optimizes all of the parameters that are important for the imaging of biological specimens in the AFM. This novel imaging chamber has been successfully tested using mammalian, plant, and microbial cells. Microsc. Res. Tech. 76:357–363, 2013. © 2013 Wiley Periodicals, Inc. |
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Keywords: | AFM mechanical properties fluid cell Arabidopsis thaliana macrophage E. coli |
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