MRT letter: Localization of endogenous hydrogen peroxide by modified processes of sample preparation for transmissionelectron microscope in Escherichia coli |
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| Authors: | Xin Li Rongliu Hu Wenxue Zhu Jinling Fan Xinyue Pang Na Wang Liping Wang Lipeng Yang Chunyan Zhao Chenyang He |
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| Affiliation: | 1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China;2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;3. College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471003, China;4. School of Pharmacy, Lanzhou University, Lanzhou 730000, China |
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| Abstract: | The bacterial endogenous hydrogen peroxide (H2O2) was detected cytochemically by its reaction with cerium chloride (CeCl3) to produce electron‐dense deposits of cerium perhydroxides. The sequence of fixation and CeCl3 staining of H2O2 in the processing of transmission electron microscope (TEM) sample preparation is crucial to the localization of endogenous H2O2 in Escherichia coli. In this study, results confirmed that the process that fixation simultaneously with CeCl3 staining provided optimum effects for H2O2 localization in E. coli. The modified process of TEM provides very efficient protection for H2O2 localization and more accurate quantization for the H2O2 accumulation in bacterial cells. Microsc. Res. Tech., 2013. © 2012 Wiley Periodicals, Inc. |
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| Keywords: | Fixation hydrogen peroxide staining transmission electron microscope Escherichia coli |
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