不同工艺提取葡萄籽油对体内抗氧化特性的影响

本文研究两种不同方法提取的葡萄籽油对衰老模型小鼠体内抗氧化功能的影响。首先采用纤维素酶浸提和超声波辅助提取葡萄籽油,精炼后灌胃由D-半乳糖诱导的亚急性衰老模型小鼠,测定小鼠心脏、肝、脑和血清中T-AOC、SOD、GSH-Px和MDA的含量。结果显示,与空白对照组相比,模型组小鼠心脏、肝、脑和血清中T-AOC、SOD、GSH-Px均有不同程度的降低,MDA含量有不同程度的升高,说明小鼠衰老模型构建成功;与模型组相比,两种葡萄籽油各剂量组均能对小鼠脑、肝、心脏和血清中的T-AOC、SOD、GSH-Px活性均有不同程度增强,对MDA值有不同程度的降低;纤维素酶辅助提取的葡萄籽油在增强小鼠体内总抗氧化能力和增强小鼠血清中GSH-Px活力方面稍优于超声波提取的葡萄籽油。结果表明,葡萄籽油能够显著增强衰老小鼠的抗氧化能力;纤维素酶辅助提取的葡萄籽油体内抗氧化能力稍强于超声波辅助法所提葡萄籽油。

Effects of Two Extraction Techniques on in vivo Antioxidant Activity of Grape Seed Oil

In this study, the effects of two extraction methods on in vivo antioxidant activity in a mouse model for aging were evaluated. First, cellulase-assisted and ultrasound-assisted extractions were used to obtain grape seed oil, which was refined and administered by gavage to the aging mice (D-galactose-induced). The contents of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione and peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in the cerebrum, heart, liver, and serum of the mice were measured. The results showed that the contents of T-AOC, SOD, and GSH-Px in the cerebrum, heart, liver, and serum of the model group all showed different degrees of reduction and the MDA content increased to different degrees, indicating that the mouse model for aging was successfully established. Compared to the model group, each dose group of two kinds of extracted grape seed oil could both enhance the activities of T-AOC, SOD, and GSH-Px and reduce MDA content in the serum and cerebrum, heart, and liver tissues to various degrees. The grape seed oil obtained using cellulase-assisted extraction was slightly superior to that obtained using ultrasound-assisted extraction in terms of enhancing T-AOC and GSH-Px activities. These findings demonstrated that grape seed oil could obviously improve antioxidant activity in the mice; the in vivo antioxidant activity of grape seed oil obtained using cellulase-assisted extraction was slightly higher than that of the grape seed oil obtained using ultrasound-assisted extraction.