NASBA技术及其在检验检疫中的应用

序列特异性核酸体外扩增技术(nucleic acid specific-based amplification,NASBA)是在PCR基础上发展起来的一种扩增RNA的新技术,作为一种新型研究手段,具有便捷、准确性好、灵敏度高、周期短的特点,尤其适用于RNA的分析研究。核酸分析技术是出入境检验检疫工作的重要手段,可用于食品病原微生物、动植物产品中的有害生物、病原的分析及鉴定。本文简要介绍了NASBA技术的基本原理,在论证对比NASBA技术与普通PCR方法、荧光PCR方法及其他恒温扩增等核酸分析技术的差异及相似性后,根据其使用特点进一步对NASBA在进出境检验检疫的食品安全检测及动植物检疫的应用予以综述及展望。

Nucleic acid specific-based amplification and its application in inspection and quarantine

Nucleic acid specific-based amplification (NASBA) is a new technology to amplify RNA originated in PCR. As a new research means, NASBA has the characteristics of convenience, good accuracy, high sensitivity and short periods, especially applicable to RNA analysis. The nucleic acid analysis technology is an important means in entry-exit inspection and quarantine, which can be used to detect and identify pathogenic microorganism in food, pests and pathogen in animals and plants. The paper briefly introduced basic principle of NASBA, compared NASBA to RT-PCR, realtime RT-PCR and other isothermal amplification methods and revealed their differences and similarity. According to the usage characteristics of NASBA, the paper reviewed the application and prospect of NASBA in food safety detection and animal and plant quarantine in entry-exit inspection and quarantine.