| 嗜麦芽寡养单胞菌发酵产聚羟基烷酸酯的研究 |
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| 引用本文： | 李雪云,付时雨,李辉,詹怀宇. 嗜麦芽寡养单胞菌发酵产聚羟基烷酸酯的研究[J]. 广东化工, 2010, 37(11): 7-9,12 |
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| 作者姓名： | 李雪云 付时雨 李辉 詹怀宇 |
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| 作者单位： | 华南理工大学,制浆造纸工程国家重点实验室,广东,广州,510640;华南理工大学,制浆造纸工程国家重点实验室,广东,广州,510640;华南理工大学,制浆造纸工程国家重点实验室,广东,广州,510640;华南理工大学,制浆造纸工程国家重点实验室,广东,广州,510640 |
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| 基金项目： | 国家自然科学基金,国家973项目 |
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| 摘 要： | 嗜麦芽寡养单胞菌(Stenotrophomonas maltophlia)HG-B-1是一株可产PHAs的菌株。文章采用逐因子试验和响应面分析法优化S.maltophlia HG-B-1的发酵工艺条件。首先考察了菌株HG-B-1的生长曲线,得到其生长周期约为24h。在此基础上,运用逐因子试验确定其生长的最适碳源和氮源,分别为麦芽糖和NH4Cl;然后在单因素试验的基础上采用三因素五水平的响应面分析,根据回归分析确定较优的发酵工艺条件为:温度35.4℃,pH7.9,摇床转速129rmin-1。采用该工艺条件发酵24h,得到OD600为2.966,是理论值的98.8%。
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| 关 键 词： | 嗜麦芽寡养单胞菌 发酵 聚羟基烷酸酯 响应面分析 |
Optimization of Polyhydroxyalkanoates Production Conditions for Stenotrophomonas Maltophlia HG-B-1 |
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| Li Xueyun,Fu Shiyu,Li Hui,Zhan Huaiyu. Optimization of Polyhydroxyalkanoates Production Conditions for Stenotrophomonas Maltophlia HG-B-1[J]. Guangdong Chemical Industry, 2010, 37(11): 7-9,12 |
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| Authors: | Li Xueyun Fu Shiyu Li Hui Zhan Huaiyu |
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| Affiliation: | Li Xueyun,Fu Shiyu,Li Hui,Zhan Huaiyu(State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,China) |
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| Abstract: | Stenotrophomonas maltophlia HG-B-1 was a strain for polyhydroxyalkanoates producing.Fermentation condition of S.maltophlia HG-B-1 was optimized by seriatim-factorial experiment,response surface methodology and monofactorial experiment.The optimum carbon and nitrogen for HG-B-1 were obtained by seriatim-factorial experiment,which were maltose and NH4Cl respectively.Experiment factors and levels were firstly selected by one-factor test,then central-composite design and response surface analysis with three fac... |
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| Keywords: | Stenotrophomonas maltophlia fermentation polyhydroxyalkanoates response surface analysis (RSA) |
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