DNA‐SMART: Biopatterned Polymer Film Microchannels for Selective Immobilization of Proteins and Cells |
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| Authors: | Ann‐Kathrin Schneider Pavel M Nikolov Stefan Giselbrecht Christof M Niemeyer |
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| Affiliation: | 1. Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann‐von‐Helmholtz‐Platz, Eggenstein‐Leopoldshafen, Germany;2. MERLN Institute for Technology‐Inspired Regenerative Medicine, Department of Complex Tissue Regeneration, Maastricht University, MD, Maastricht, The Netherlands |
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| Abstract: | A novel SMART module, dubbed “DNA‐SMART” (DNA substrate modification and replication by thermoforming) is reported, where polymer films are premodified with single‐stranded DNA capture strands, microthermoformed into 3D structures, and postmodified with complementary DNA‐protein conjugates to realize complex biologically active surfaces within microfluidic devices. As a proof of feasibility, it is demonstrated that microchannels presenting three different proteins on their inner curvilinear surface can be used for selective capture of cells under flow conditions. |
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| Keywords: | cells DNA fluidics microstructures proteins |
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