Prenatal and postnatal diagnoses of thalassemias and hemoglobinopathies by HPLC |
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Authors: | S Fucharoen P Winichagoon R Wisedpanichkij B Sae-Ngow R Sriphanich W Oncoung W Muangsapaya J Chowthaworn S Kanokpongsakdi A Bunyaratvej A Piankijagum C Dewaele |
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Affiliation: | Thalassemia Research Center, Department of Medicine, Mahidol University, Nakornpathom, Thailand. grsfc@mahidol.ac.th |
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Abstract: | The conventional approach to qualitative and quantitative analyses of hemoglobin (Hb) molecules for the diagnoses of hemoglobinopathies requires a combination of tests. We used an automated HPLC (VARIANT) system to study alpha-thalassemia and beta-thalassemia syndromes in Thailand. The beta-thalassemia short program is applicable to the diagnosis of alpha-thalassemia and beta-thalassemia disorders, including Hb H, EA Bart's disease, and EF Bart's disease, in adults, newborns, and fetuses. The system cannot quantify accurately certain Hb molecules, such as Hb H and Hb Bart's. The alpha-thalassemia short program was therefore developed and used to quantify Hb Bart's to detect alpha-thalassemia genotypes in cord blood. This automated HPLC system is an alternative approach to the diagnosis of complicated thalassemia syndromes in Thailand and Southeast Asia. |
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