Direct electrochemistry of chemically modified catalase immobilized on an oxidatively activated glassy carbon electrode |
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Authors: | Sedigheh Hashemnia Hedayatollah Ghourchian Ali Aakbar Moosavi-Movahedi Hassan Faridnouri |
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Affiliation: | (1) Persian Gulf University, Bushehr, 75169, Iran;(2) Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran |
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Abstract: | Catalase (Ct) was modified using Woodward’s reagent K (WRK) as a specific modifier of carboxyl residues. The modified Ct was
immobilized on an oxidatively activated glassy carbon electrode surface to investigate its direct electrochemistry. Using
cyclic voltammetry an irreversible reduction peak was obtained at approximately −0.362 V vs. Ag/AgCl in buffer solution, pH
7, and at a scan rate of 0.1 V s−1. The electrochemical parameters, including charge-transfer coefficient (0.27), apparent heterogeneous electron transfer rate
constant (13.51 ± 0.42 s−1) and formal potential of the Ct film (−0.275 V) were determined. The prepared enzyme electrode exhibited a response to H2O2. |
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Keywords: | Catalase Direct electrochemistry Chemical modification Woodward’ s reagent K Hydrogen peroxide |
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