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| 啤酒泡沫活性蛋白质中Z4蛋白质的提取及鉴定 | |
| 引用本文： | 贾娟,王德良,傅力,王建. 啤酒泡沫活性蛋白质中Z4蛋白质的提取及鉴定[J]. 食品与发酵工业, 2007, 33(2): 133-136 |
| 作者姓名： | 贾娟 王德良 傅力 王建 |
| 作者单位： | 1. 新疆农业大学食品科学学院,新疆乌鲁木齐,830052, 2. 中国食品发酵工业研究院,北京,100027, 3. 北京化工大学生命科学与技术学院,北京,100029 |
| 摘 要： | 主要研究了泡沫活性蛋白质中最重要的Z4蛋白的分离提纯,并利用蛋白免疫印记法(Western Blot)以及质谱(Mass Spectrometry)鉴定Z4蛋白质。此外,还探讨了Z4蛋白质的含量在酿造过程中的变化。实验结果表明,Z4蛋白质的pI值为5.5~6.0,分子质量为43ku,其抗体鉴定Z4有很高的相关性。在酿造过程中,泡沫活性蛋白质发生了很大的变化。 |
| 关 键 词： | 啤酒 泡沫活性蛋白质 Z4蛋白质 免疫印记法 质谱 |
| 修稿时间： | 2006-10-25 |
Isolation and Identification of Protein Z4 in Beer Foam-positive Protein |
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| Jia Juan,Wang Deliang,Fu Li,Wang Jian. Isolation and Identification of Protein Z4 in Beer Foam-positive Protein[J]. Food and Fermentation Industries, 2007, 33(2): 133-136 | |
| Authors: | Jia Juan Wang Deliang Fu Li Wang Jian |
| Affiliation: | 1.College of Food Science, Xinjiang Agriculture University, Urumqi 830052, China;2.China National Institute of Food and Fermentation Industries,Beijing 100027,China;3.College of Life Science and Teehnology,Beijing University of Chemical Technology, Beijing 100029,China |
| Abstract: | In this paper,we mainly studied the method of isolation of protein Z4, a foam-posirive protein of critical importance in beer, and the application of immunobloting for detection of protein Z4. Furthermore, the change of content of protein Z4 was also analyzed through the process of brewery production. The results showed that the pI value amounted to 5.5-6.0 and its molecular weight is 43000 in protein Z4,which is highly relevant to Z4 antibody indentification. There is great change in protein Z4 through the process of brewery production. |
| Keywords: | beer foam-positive protein protein Z4 Western Blot Mass Spectrometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |