| 发酵罐中生产纳豆激酶的条件与动力学研究 |
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| 引用本文： | 沙维,刘妍妍,张丽萍. 发酵罐中生产纳豆激酶的条件与动力学研究[J]. 食品与发酵工业, 2010, 0(2) |
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| 作者姓名： | 沙维 刘妍妍 张丽萍 |
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| 作者单位： | 黑龙江八一农垦大学食品学院;黑龙江省农产品加工工程技术研究中心; |
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| 基金项目： | 黑龙江省农垦总局科技计划项目(HNKXIV-06-05a); 黑龙江省研究生创新科研项目(YJSCX2009-125HLJ) |
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| 摘 要： | 对纳豆杆菌进行了10L发酵罐分批发酵试验,研究发酵罐装液量、接种量、搅拌桨转速对纳豆激酶发酵的影响,并采用Logistic方程、Luedking-Piret方程、Luedking-Piret-like方程描述了发酵中菌体生长、纳豆激酶生成、基质消耗特性。在装液量5L、接种量3%、搅拌桨转速400r/min条件下,发酵得到纳豆激酶活力4 476.25IU/mL,分批发酵过程的动力学模型与实验值拟合度较好。
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| 关 键 词： | 纳豆激酶 液态发酵 优化 动力学 |
Study on Conditions and Kinetics of Nattokinase Fermentation in Fermentor |
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| Sha Wei,Liu Yanyan,Zhang Liping. Study on Conditions and Kinetics of Nattokinase Fermentation in Fermentor[J]. Food and Fermentation Industries, 2010, 0(2) |
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| Authors: | Sha Wei Liu Yanyan Zhang Liping |
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| Affiliation: | Sha Wei1,Liu Yanyan1,Zhang Liping1,2 1(College of Food Science,Heilongjiang Bayi Agricultural University.Daqing,Heilongjiang 163319,China) 2(Agri-Food Processing , Engineering Technology Research Center of Heilongjiang Province,Daqing,China) |
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| Abstract: | The fermentation process of Bacillus natto was studied in a 10-L batch system.The fermentation conditions including volume of the medium,inoculation quantity and rotation speed.Models were proposed with the Logistic equation for growth,the Luedking-Piret equation for nattokinase production and Luedking-Piret-like equation for substrate consumption.The optimized conditions were as follows: liquid volume 5L/10L,rotation speed 400r/min,3% inoculum.Under these conditions the activity of nattokinase was 4 476.25... |
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| Keywords: | nattokinase liquid fermentation optimization kinetic |
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