超高效液相色谱串联质谱法检测水稻中五氟磺草胺、吡嘧磺隆和丙草胺的残留量

目的]建立了以QuEChERS为样品前处理方法的超高效液相色谱串联质谱(UPLC-MS/MS)同时测定水稻中五氟磺草胺、吡嘧磺隆和丙草胺残留量的分析方法.方法]样品经乙腈提取,C18净化,电喷雾电离、正离子模式采集,多反应监测模式检测,基质匹配标准品外标法定量.结果]五氟磺草胺、吡嘧磺隆和丙草胺的进样质量浓度与对...

Determi nation of the Residues of Peno xsulam,Pyrazosulfur on-ethyl and Pretilachlor in Rice by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

Aims]This study aims to establish a method for the simultaneous residue determination of penoxsulam,pyrazosulfuron-ethyl and pretilachlor in rice based on QuEChERS(quick,easy,cheap,effective,rugged and safe)and ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods]The samples were extracted with acetonitrile,and purified with octadecylsilane(C₁₈).The mass spectrometer was performed in multi-reaction monitoring mode,and electrospray ionization in positive mode was selected.The penoxsulam,pyrazosulfuron-ethyl and pretilachlor residues were quantified by the external standard method using the matched standard solution to compensate for matrix effect.Results]The results showed that there was a good linear correlation between the concentrations of penoxsulam,pyrazosulfuron-ethyl and pretilachlor,their peak areas with correlation coefficients of more than 0.9926.The average recoveries of the three analytes varied from 88 to 111%with the relative standard deviation(RSD)ranging from 0.9 to 9%in brown rice,rice husk and rice plants.Conclusions]This method is rapid,simple and accurate,and can be adapted for the confirmation of penoxsulam,pyrazosulfuron-ethyl and pretilachlor residues in rice samples.The results of twelve locations revealed that the penoxsulam,pyrazosulfuron-ethyl and pretilachlor in rice was lower than the maximum residue limit standard for brown rice set by China.