In vitro studies on calf thymus DNA interaction and 2-tert-butyl-4-methylphenol food additive |
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Authors: | Soheila Kashanian Jafar Ezzati Nazhad Dolatabadi |
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Affiliation: | (1) Faculty of Chemistry, Nanoscience and Nanotechnology Research Center (NNRC), Razi University, Kermanshah, I.R. Iran;(2) Department of Biology, Faculty of Science, Razi University, Kermanshah, I.R. Iran |
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Abstract: | The interaction of native calf thymus DNA (CT-DNA) with 2-tert-butyl-4-methylphenol (TBMP) at physiological pH has been investigated by spectrofluorometric and viscosimetric techniques.
TBMP molecules were found to intercalate between base pairs of DNA, demonstrated by an increase in the specific viscosity
of DNA and decrease in the fluorescence of TBMP solutions in the presence of increasing amounts of DNA and the calculated
binding constants (K
f) at different temperatures. Furthermore, the enthalpy and entropy of the reaction between TBMP and CT-DNA showed that the
reaction is exothermic and enthalpy favored (ΔH = −19.18 kJ mol−1; ΔS = −26.98 J mol−1 K−1) which are other evidences to indicate that TBMP is able to be intercalated in the DNA base pairs. |
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