共轭亚油酸对缺氧损伤的保护作用

为了探讨共轭亚油酸(CLA)对缺氧损伤的保护作用,40只SD大鼠随机分为4个组:1%CLA组、1%CLA缺氧组、空白对照组和空白缺氧组。CLA组和空白对照组用1%CLA和蒸馏水分别连续灌胃4周,剂量为20ml/kg/d。灌胃结束后,1%CLA缺氧组和空白缺氧组大鼠在5000m海拔高度下,连续缺氧15d,每天8h,然后测定各组大鼠脏器/体重比、血清SOD和GSH-PX活性以及MDA含量。结果发现,在缺氧条件下,大鼠脏器/体重比显著增大(p<0.05),SOD和GSH-PX活性显著下降(p<0.05),MDA含量明显升高(p<0.05)。1%CLA缺氧组大鼠脏器/体重比、血清SOD和GSH-PX活性以及MDA含量与正常大鼠无明显差异(p>0.05)。上述结果说明,1%CLA对缺氧损伤具有明显的保护作用。

Protective Role of Conjugated Linoleic Acid to Hypoxia Injury in Rats

To explore the protective role of conjugated linoleic acid (CLA) in hypoxia injury, forty SD rats were divided randomly into four groups, including positive CLA groups (1%CLA group, 1%CLA hypoxia group) and negative control groups (control group, control hypoxia group). Both the positive CLA groups and negative control groups were fed with 1%CLA and distilled water respectively and each animal received a constant daily dose of 20ml/kg body weight for four weeks consecutively. After the treatment, the hypoxic groups were exposed eight hours daily at the altitude of five thousand meters for fifteen consecutive days. The ratios of activities of SOD, GSH-PX and the contents of MDA in serum were determined respestively. The results showed that, under the hypoxic condition, the ratio of viscera weight to body weight increased obviously(p<0.05). While the activities of SOD and GSH-PX decreased markedly(p<0.05) and the contents of MDA increased significantly(p<0.05) in rat serum. The ratio of viscera weight to body weight, the activities of SOD, GSH-PX, and the contents of MDA in 1% CLA hypoxic group showed no obvious difference in comparison with that of control group. In conclusion, 1%CLA had obvious protectiver olei nh ypoxia injury.