鸡膝软骨胶原蛋白肽的超声辅助酶提工艺优化及抗氧化性

研究超声波辅助酶提取鸡膝软骨胶原蛋白肽的工艺,确定最佳实验用酶为碱性蛋白酶.以料液比、超声功率和酶提时间为试验因素,胶原蛋白肽得率为响应值,利用Box-Behnken试验和响应面法分析试验,确定最佳提取工艺条件.结果表明:最佳提取工艺参数为料液比1:26(m/V)、超声功率253?W、酶提时间4?h,此时鸡膝软骨胶原蛋...

Optimization of Ultrasound-Assisted Enzymatic Extraction of Collagen Peptide from Chicken Knee Cartilage and Its Antioxidant Activity

The ultrasound-assisted enzymatic extraction of collagen peptide from chicken knee cartilage was studied in this work. Alkaline protease was selected as the optimum enzyme. In an effort to determine the optimal processing conditions using Box-Behnken design combined with response surface methodology (RSM), solid-to-solvent ratio, ultrasonic power and extraction time were considered as the independent variables, and the yield of collagen peptide was considered as the response. A solid-to-solvent ratio of 1:26 (m/V), an ultrasonic power of 253 W and enzymatic extraction for 4 h were found to be the optimal conditions, under which the experimentally measured yield of collagen peptide was 42.83%. This experimental value was in good agreement with the predicted one (43.32%) with a relative error of only 0.49%. Antioxidant tests showed that the half maximal inhibitory concentration (IC50) of the collagen peptide prepared using the optimized conditions was 8 and 8.5 mg/mL for scavenging of 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate) cation radical and hydroxyl radical, respectively. The collagen peptide, despite having potent metal ion chelating ability, exhibited lower reducing power than did VC.