固相萃取结合超高效液相色谱-质谱法测定畜禽肉及肉制品中肾上腺素和多巴胺的残留量

建立畜禽肉及肉制品中2 种儿茶酚胺类化合物（肾上腺素和多巴胺）的超高效液相色谱-质谱联用检测方法。样品采用10 mmol/L磷酸二氢钾溶液提取，WCX固相萃取柱净化，Waters Acquity BEH C18柱分离，采用电喷雾离子源，正离子多反应模式监测，基质匹配内标法定量。结果表明：肾上腺素和多巴胺在0.5～50 ng/mL范围内，相关系数（R2）均大于0.996；在畜肉中的检出限和定量限分别为0.39～0.40 μg/kg和1.00 μg/kg，在禽肉中的检出限和定量限分别为0.36～0.37 μg/kg和0.98 μg/kg，在肉制品中的检出限和定量限分别为0.38 μg/kg和0.96～0.97 μg/kg；加标量为1～10 μg/kg时，方法回收率为83.7%～111.2%，相对标准偏差为1.28%～5.76%。该方法具有灵敏度高、定量准确等优点，适用于畜禽肉及肉制品中残留儿茶酚胺类化合物含量的测定。

Determination of Epinephrine and Dopamine Residues in Meat and Meat Products by Solid Phase Extraction Combined with Ultra-High Performance Liquid Chromatography-Mass Spectrometry

An ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) method was established for the detection of the residues of two catecholamine compounds (epinephrine and dopamine) in meat and meat products. The samples were extracted with 10 mmol/L phosphate buffer solution, and the extract was purified on a WCX solid phase extraction column, and separated on a Waters Acquity BEH C18 column. The analytes were detected using an electrospray ion source in the positive ion multiple reaction monitoring (MRM) mode, and quantified by a matrix matched internal standard method. The results showed that the calibration curves for epinephrine and dopamine were linear in the concentration range of 0.5–50 ng/mL with correlation coefficient (R2) greater than 0.996. The limits of detection and limits of quantification were 0.39–0.40 and 1.00 μg/kg for livestock meat, 0.36–0.37 and 0.98 μg/kg for poultry meat, and 0.38 and 0.96–0.97 μg/kg for meat products, respectively. At spiked concentrations of 1–10 μg/kg, the recoveries of the method were between 83.7% and 111.2%, and the precision relative standard deviation (RSD) was 1.28%–5.76%. This method has the advantages of high sensitivity and accuracy, and is suitable for the determination of residual catecholamine compounds in meat and meat products.