高效液相色谱-串联质谱法测定猪肉及猪肉制品中牛磺酸含量

建立猪肉及猪肉制品中牛磺酸含量的高效液相色谱-串联质谱(high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)检测方法.样品经水超声提取后,经乙腈沉淀蛋白,正己烷除脂肪,采用HPLC-MS/MS法定量测定.结果 表明:在1～...

Determination of Taurine in Pork and Pork Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry

This study intended to develop a method for rapid determination of taurine in pork and pork products by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The samples were ultrasonically extracted with water,added with acetonitrile to precipitate protein,and re-extracted with n-hexane to remove fat.Then the analytes were detected by HPLC-MS/MS.The matrix-matched calibration curve developed showed a good linearity within the concentration range from 1 to 1000 ng/mL(R²>0.998).The average recovery for positive samples spiked with taurine at concentration levels of 20–50 mg/100 g was from 92.78%to 104.96%,with relative standard deviation(RSD)between 1.39%and 3.80%.The instrumental detection limit and quantitation limit were 0.3 ng/mL and 1 ng/mL respectively;the method detection limit and quantitation limit were 0.1 mg/100 g and 0.5 mg/100 g respectively.The method is characterized by low detection limit,as well as high recovery,sensitivity,accuracy and precision.It is simple and quick to operate and suitable for the accurate determination of taurine in pork and pork products.