| 乌珠穆沁羊、杜泊羊和萨福克羊脂肪酸指纹图谱的建立 |
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| 引用本文： | 苏 馨,车天宇,谢遇春,赵 存,郭俊涛,王志新,包永泉,李金泉,刘志红.乌珠穆沁羊、杜泊羊和萨福克羊脂肪酸指纹图谱的建立[J].肉类研究,2021,35(8):37. |
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| 作者姓名： | 苏 馨 车天宇 谢遇春 赵 存 郭俊涛 王志新 包永泉 李金泉 刘志红 |
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| 作者单位： | 1.内蒙古农业大学动物科学学院，内蒙古自治区动物遗传、育种和繁殖重点实验室，农业部肉羊遗传育种重点实验室，内蒙古自治区山羊遗传育种工程技术研究中心，内蒙古 呼和浩特 010018；2.东乌珠穆沁旗动物疫病预防控制中心，内蒙古 锡林郭勒 026000 |
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| 基金项目： | 内蒙古自治区关键技术攻关计划项目（2020GG0030）；内蒙古自治区科技重大专项（2020ZD0004）；国家自然科学基金地区项目（32060742）；内蒙古自治区双一流建设项目青年基金项目（QN202001） |
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| 摘 要： | 挑选内蒙古地区相同饲喂条件下的乌珠穆沁羊、杜泊羊和萨福克羊各6 只，取背最长肌，通过气相色谱-质谱进行脂肪酸检测，构建指纹图谱，利用中药色谱指纹图谱相似度评价系统软件进行指纹图谱相似度分析和主成分分析。结果表明：杜泊羊和萨福克羊背最长肌脂肪酸指纹图谱相似度为0.991，杜泊羊和乌珠穆沁羊背最长肌脂肪酸指纹图谱相似度为0.987，萨福克羊和乌珠穆沁羊背最长肌脂肪酸指纹图谱相似度为0.994。3 种羊背最长肌脂肪酸指纹图谱中共存在12 个共有峰，以硬脂酸（C18:0）色谱峰为参比峰，对12 个共有峰的保留时间和相对峰面积进行主成分分析，可准确鉴别以上3 个品种羊。
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| 关 键 词： | 脂肪酸 指纹图谱 乌珠穆沁羊 杜泊羊 萨福克羊 相似度 |
Fatty Acid Fingerprinting of Wuzhumuqin,Dorper and Suffolk Sheep |
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| SU Xin,CHE Tianyu,XIE Yuchun,ZHAO Cun,GUO Juntao,WANG Zhixin,BAO Yongquan,LI Jinquan,LIU Zhihong.Fatty Acid Fingerprinting of Wuzhumuqin,Dorper and Suffolk Sheep[J].Meat Research,2021,35(8):37. |
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| Authors: | SU Xin CHE Tianyu XIE Yuchun ZHAO Cun GUO Juntao WANG Zhixin BAO Yongquan LI Jinquan LIU Zhihong |
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| Affiliation: | 1.The Inner Mongolia Autonomous Region Goat Genetics and Breeding Engineering Technology Research Center, Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. Eastern Wuzhumuqin Animal Disease Prevention and Control Center, Xilingol 026000, China |
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| Abstract: | In this experiment, six Wuzhumuqin, Dorper and Suffolk sheep raised under the same conditions in Inner Mongolia each were selected and slaughtered. Longissimus dorsi muscles were taken for fatty acid fingerprinting by gas chromatography-mass spectrometry (GC-MS). Similarity analysis of the developed fingerprints was carried out using the software of similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (TCM) and principal component analysis (PCA) was performed as well. The results showed that the similarity in the fatty acid fingerprint of Longissimus dorsi was 0.991 for Dorper versus Suffolk sheep, 0.987 for Dorper versus Wuzhumuqin sheep, and 0.994 for Suffolk versus Wuzhumuqin Sheep. A total of 12 peaks were common to the fatty acid fingerprints of Longissimus dorsi of the three breeds. Based on the retention times of the 12 common peaks and the relative peak areas, PCA accurately discriminated these breeds using stearic acid (C18:0) as the reference peak. |
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| Keywords: | fatty acids fingerprint Wuzhumuqin sheep Dorper sheep Suffolk sheep similarity |
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